Silica Gel Column Chromatography

Thin-Layer Chromatography- An Introduction There are many different types of chromatography in use to separate components of various mixtures. One of the most commonly used is Thin Layer Chromatography or TLC, as it is popularly known as. Thin Layer Chromatography is mainly employed to separate non-volatile mixtures. This form of chromatography works by coating a glass sheet, plastic or aluminium foil with adsorbent material like Silica Gel, Aluminium Oxide and Cellulose and then passing the mixture through it. This mixture is generally known as the mobile phase. Depending on their polarity, different components in the mixture, which is to be separated will be absorbed at different levels. This is how the Thin Layer Chromatography process works. Now, Column Chromatography Another form of chromatography, which is equally popular is column chromatography. It is known as column chromatography because the separation process takes place in a vertical glass column. In this process, ...