The Specific Uses of Thin Layer Chromatography
Thin layer Chromatography is a process of first separating the compounds in a mixture and the suitably analyzing each one of them. The identity of each compound can be established along with their purity or concentration levels in the mixture. TLC chromatography is a very sensitive method and even the most micro quantities can be analyzed by this process. The time required for this separation and subsequent analysis may take between 5 – 10 minutes. The Process Involved in Thin layer Chromatography Generally, a three phased procedure is involved in the TLC. Firstly, the substance that needs to be investigated is dissolved in a volatile solvent that is easily evaporated that produces a very dilute solution. All along the separation process, photographs are taken regularly at all the different stages, which are often three in nature. The first stage consists of spotting that uses a micro pipette in which a very small amount of the diluted solution is added and exposed