Silica Gel Column Chromatography

Chromatography is a physical process, used to analyze the trace amounts of chemicals from a complex mixture. Chromatography is a valuable technique and gives accurate results, mainly because of the Silica Gel absorbent. In Chromatography, the most commonly used is Column Chromatography Technique. To avoid the loss of solvents volume and for reasonable separation, Adsorbent Silica gel chromatography is in great demand. Since Silica Gel has a closely controlled pore size, it allows the components of complex mixture to pass through the absorbent, based on the affinity. Strongly absorbed component by the Silica Gel remains on the top of the column, where as remaining components flow down in the Silica Gel column. About Silica gel Column Chromatography Silica Gel grades called as mesh, act as barrier and helps in determining the amount of single component present in a complex mixture. This Silica gel mesh absorbent is available with different labels based on the mesh size. The

Thin-Layer Chromatography- An Introduction There are many different types of chromatography in use to separate components of various mixtures. One of the most commonly used is Thin Layer Chromatography or TLC, as it is popularly known as. Thin Layer Chromatography is mainly employed to separate non-volatile mixtures. This form of chromatography works by coating a glass sheet, plastic or aluminium foil with adsorbent material like Silica Gel, Aluminium Oxide and Cellulose and then passing the mixture through it. This mixture is generally known as the mobile phase. Depending on their polarity, different components in the mixture, which is to be separated will be absorbed at different levels. This is how the Thin Layer Chromatography process works. Now, Column Chromatography Another form of chromatography, which is equally popular is column chromatography. It is known as column chromatography because the separation process takes place in a vertical glass column. In this process,

The principal elements of Silica Gel are that should be an excellent adsorbent as well as has a very huge surface area. These kinds of attributes benefit it in adsorbing little impurities and allow only the desired molecules to pass through. In many these types of chromatography, the solid or stationary phase (Silica Gel) essentially adsorbs the several elements at many levels to achieve higher levels of filtration. Grade Laboratory Grade Particle Size: 70-230 Mesh (210-63μm) Boiling Point: 2230 0 C Melting Point: >1600 0 C Bulk Density KG/LIT 500-650 Ph 10% aq solution 7 ± 0.5 Fe (Iron) <75 ppm Chloride(NaCl) Max 300ppm Pore Diameter –A 40-60A Surface Area m 2 /gm 350-550