Thursday, August 10, 2017


Chromatography is a physical process, used to analyze the trace amounts of chemicals from a complex mixture. Chromatography is a valuable technique and gives accurate results, mainly because of the Silica Gel absorbent. In Chromatography, the most commonly used is Column Chromatography Technique. To avoid the loss of solvents volume and for reasonable separation, Adsorbent Silica gel chromatography is in great demand. Since Silica Gel has a closely controlled pore size, it allows the components of complex mixture to pass through the absorbent, based on the affinity. Strongly absorbed component by the Silica Gel remains on the top of the column, where as remaining components flow down in the Silica Gel column.

About Silica gel Column Chromatography

Silica Gel grades called as mesh, act as barrier and helps in determining the amount of single component present in a complex mixture. This Silica gel mesh absorbent is available with different labels based on the mesh size. The mesh of the Sieve is used to size the Silica during the manufacturing process, in which the numbering represents the number of holes of the mesh or the sieve through which crude silica is passed on. The smaller the size of the Silica gel particle the higher the mesh value, the larger the size of the Silica Gel particle the lower the mesh values. Therefore Silica gel mesh with different sizes mainly smaller silica gel particles such as 70-230 mesh is employed for gravity columns and 200-400 mesh is utilized in Flash Columns for sample extraction. Silica gel absorbent particle size affects the flow of solvent through the Column. For easy separation using Silica gel Column chromatography, 30:1 ratio of absorbent is required, whereas for difficult separations more Absorbent Silica gel is required. Don't miss this article: Best Guidelines for pharma packaging.

Silica gel mesh for Column Chromatography acts as stationery phase and therefore, separation of the Solvent is based on the degree of its absorption. Silica Gel being acidic and polar, it allows the more polar component in the mixture to remain for a longer time in the Column whereas the non-polar components in the mixture flow down and get eluted first. Chromatography is helpful in detecting errors in metabolism caused by the body fluids. Silica gel is also used in breweries to prevent haze formation due to proteins. The Silica gel being a good absorbent for proteins, it prevents haze formation without affecting the quality. Since Silica Gel products are not readily bio degradable, environmentally non-hazardous and non carcinogenic, they can be employed in all the techniques of purification and separation. The components in a mixture, having the same physical and chemical properties can only be separated by Silica Gel Chromatography method. Also check this:how to choose the packaging materials for pharma API packaging. 

Sorbead India has years of experience as a manufacturer and Silica Gel supplier for Column Chromatography techniques ,which are used for the separation and purification of synthesized products analytically. Silica Gel manufactured by Sorbead, have excellent absorption capacity and high resolution. It is also capable in providing a range of Silica Gel 60-120 mesh, Silica Gel 60-200 mesh, Silica Gel 70-230 mesh, Silica Gel 200-400 mesh and Silica Gel 400-800 mesh.

Wednesday, March 29, 2017

Thin Layer Chromatography Vs Column Chromatography

Thin-Layer Chromatography- An Introduction
There are many different types of chromatography in use to separate components of various mixtures. One of the most commonly used is Thin Layer Chromatography or TLC, as it is popularly known as. Thin Layer Chromatography is mainly employed to separate non-volatile mixtures. This form of chromatography works by coating a glass sheet, plastic or aluminium foil with adsorbent material like Silica Gel, Aluminium Oxide and Cellulose and then passing the mixture through it. This mixture is generally known as the mobile phase. Depending on their polarity, different components in the mixture, which is to be separated will be absorbed at different levels. This is how the Thin Layer Chromatography process works.
Now, Column Chromatography
Another form of chromatography, which is equally popular is column chromatography. It is known as column chromatography because the separation process takes place in a vertical glass column. In this process, the mixture is moved down the glass column and the different components are easily absorbed by the stationary phase. The rate at which the components are separated depends on their affinity to the stationary adsorbent.
Difference between Column Chromatography and Thin-Layer Chromatography
Now that we have looked at two types of chromatography, let us try to understand the basic differences between the two. These differences will help us know why one form of chromatography is preferred over the other.
1) Thin-layer chromatography requires higher samples and lower analysis time. This is because the number of samples separated via TLC is much more than that done in the column chromatography process.
2) In Thin-layer chromatography, one makes use of strong reagents to identify the different components of the mixture. This works out well in comparison to column chromatography because the TLC plate can withstand strong solvents and colour creating agents.
3) The plates used in the thin-layer chromatography process are designed in such a manner that they can be heated to very high temperatures without undergoing any damage. This may not work out for Column chromatography plates.
4) Thin-layer chromatography is said to be more sensitive as there is less spreading of substance zones in this process in comparison to the column chromatography process.

Monday, January 16, 2017

Silica Gel 70-230 Mesh

The principal elements of Silica Gel are that should be an excellent adsorbent as well as has a very huge surface area. These kinds of attributes benefit it in adsorbing little impurities and allow only the desired molecules to pass through.
In many these types of chromatography, the solid or stationary phase (Silica Gel) essentially adsorbs the several elements at many levels to achieve higher levels of filtration.

Grade Laboratory Grade
Particle Size: 70-230 Mesh (210-63μm)
Boiling Point: 2230 0C
Melting Point: >1600 0C
Bulk Density KG/LIT 500-650
Ph 10% aq solution 7 ± 0.5
Fe (Iron) <75 ppm
Chloride(NaCl) Max 300ppm
Pore Diameter –A 40-60A
Surface Area m2/gm 350-550