Silica Gel Column Chromatography

Column chromatography is now regarded as one of the very significant and widely utilized techniques for the analysis and separation of complex organic mixtures. Silica gel for carrying out column chromatography is available in different particle size desired for pilot plant application. The silica gel 200-400 mesh size is ideal for flash chromatography, which means low pressure chromatography. The very smaller size particle of 200-400 mesh size offers better separation outcome with pressurized column. This is the very commonly utilized mesh size in places like organic synthesis labs. The silica gel of mesh size 200-300 is suited for achieving gravitational chromatography. The larger particle size of this silica gel enables solvent to smoothly flow without pressure besides offering excellent separation results. In column chromatography of 100-200 silica gel grade , a solid adsorbent and the stationary phase is placed in the vertical glass column and a liquid, the mobile pha