Which Adsorbents Are Superior in Chromatography?
Chromatography and Its Different Types Chromatography is a commonly used method for the separation and purification of different mixtures. It basically involves two steps- the mobile phase and the stationary phase. The chromatography process is explained as follows- at first the mixture, which has to be separated is dissolved in the liquid mobile phase and then placed on top of a vertical glass column. Then it is passed down the vertical column containing the solid stationary phase. As the mixture makes its journey downwards, different components will get separated at different levels. This is because different components move at different speeds and this is the reason behind their separation. The latter is also dependent on the differential partitioning between the mobile and stationary phases. Chromatography is broadly divided into preparative and analytical. While preparative chromatography is used to isolate the components of any given mixture for further use, analytical