Thursday, August 10, 2017

BEST ABSOBENT FOR COLUMN CHROMATOGRAPHY

Chromatography is a physical process, used to analyze the trace amounts of chemicals from a complex mixture. Chromatography is a valuable technique and gives accurate results, mainly because of the Silica Gel absorbent. In Chromatography, the most commonly used is Column Chromatography Technique. To avoid the loss of solvents volume and for reasonable separation, Adsorbent Silica gel chromatography is in great demand. Since Silica Gel has a closely controlled pore size, it allows the components of complex mixture to pass through the absorbent, based on the affinity. Strongly absorbed component by the Silica Gel remains on the top of the column, where as remaining components flow down in the Silica Gel column.

About Silica gel Column Chromatography

Silica Gel grades called as mesh, act as barrier and helps in determining the amount of single component present in a complex mixture. This Silica gel mesh absorbent is available with different labels based on the mesh size. The mesh of the Sieve is used to size the Silica during the manufacturing process, in which the numbering represents the number of holes of the mesh or the sieve through which crude silica is passed on. The smaller the size of the Silica gel particle the higher the mesh value, the larger the size of the Silica Gel particle the lower the mesh values. Therefore Silica gel mesh with different sizes mainly smaller silica gel particles such as 70-230 mesh is employed for gravity columns and 200-400 mesh is utilized in Flash Columns for sample extraction. Silica gel absorbent particle size affects the flow of solvent through the Column. For easy separation using Silica gel Column chromatography, 30:1 ratio of absorbent is required, whereas for difficult separations more Absorbent Silica gel is required.

Silica gel mesh for Column Chromatography acts as stationery phase and therefore, separation of the Solvent is based on the degree of its absorption. Silica Gel being acidic and polar, it allows the more polar component in the mixture to remain for a longer time in the Column whereas the non-polar components in the mixture flow down and get eluted first. Chromatography is helpful in detecting errors in metabolism caused by the body fluids. Silica gel is also used in breweries to prevent haze formation due to proteins. The Silica gel being a good absorbent for proteins, it prevents haze formation without affecting the quality. Since Silica Gel products are not readily bio degradable, environmentally non-hazardous and non carcinogenic, they can be employed in all the techniques of purification and separation. The components in a mixture, having the same physical and chemical properties can only be separated by Silica Gel Chromatography method.

Sorbead India has years of experience as a manufacturer and Silica Gel supplier for Column Chromatography techniques ,which are used for the separation and purification of synthesized products analytically. Silica Gel manufactured by Sorbead, have excellent absorption capacity and high resolution. It is also capable in providing a range of Silica Gel 60-120 mesh, Silica Gel 60-200 mesh, Silica Gel 70-230 mesh, Silica Gel 200-400 mesh and Silica Gel 400-800 mesh.

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